plasmid transfection using fugene hd Search Results


peyfp-n3-parg plasmid mixed with fugene hd transfection reagent  (Promega)
90
Promega peyfp-n3-parg plasmid mixed with fugene hd transfection reagent
Peyfp N3 Parg Plasmid Mixed With Fugene Hd Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+transfection+using+fugene+hd/pm36076593-258-9-17?v=Promega
Average 90 stars, based on 1 article reviews
peyfp-n3-parg plasmid mixed with fugene hd transfection reagent - by Bioz Stars, 2026-07
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plasmids transfected with fugene hd  (Promega)
90
Promega plasmids transfected with fugene hd
Plasmids Transfected With Fugene Hd, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+transfection+using+fugene+hd/pmc07081352-295-10-12?v=Promega
Average 90 stars, based on 1 article reviews
plasmids transfected with fugene hd - by Bioz Stars, 2026-07
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plasmid expressing rabv gmyc and flaghbst2 complexed with fugene hd transfection reagent  (Promega)
90
Promega plasmid expressing rabv gmyc and flaghbst2 complexed with fugene hd transfection reagent
(A) HEK293T cells were transfected with plasmids expressing flag-hBST2. Cell lysates were prepared and incubated in the presence or absence of PNGase F and subjected to western blot analysis of hBST2. HEK293T cells were transfected with (B) 1 μg of flag-hBST2 plasmid and an increasing amount (0.5, 1 and 2 μg) of plasmids encoding RABV G-myc, M-myc, N-myc and P-myc or (C) 1 μg of plasmids encoding individual myc-tagged RABV proteins with an increasing amount of <t>flaghBST2</t> plasmid (0.5, 1 and 2 μg). At 48 hpt, cell lysates were collected and analyzed by western blotting for RABV protein and hBST2 expression using rabbit α-myc and α-human BST2 antibodies, respectively. Arrow heads indicate glycosylated monomeric hBST2 of approximately 30 kDa. Arrows indicate a low molecular weight, glycosylated dimeric hBST2 of approximately 55 kDa. Asterisks indicate high molecular weight glycosylated dimeric hBST2 of approximately 65 kDa.
Plasmid Expressing Rabv Gmyc And Flaghbst2 Complexed With Fugene Hd Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+transfection+using+fugene+hd/pmc10624315-119-18-26?v=Promega
Average 90 stars, based on 1 article reviews
plasmid expressing rabv gmyc and flaghbst2 complexed with fugene hd transfection reagent - by Bioz Stars, 2026-07
90/100 stars
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plasmids transfected using fugene 6  (Promega)
90
Promega plasmids transfected using fugene 6
(A) HEK293T cells were transfected with plasmids expressing flag-hBST2. Cell lysates were prepared and incubated in the presence or absence of PNGase F and subjected to western blot analysis of hBST2. HEK293T cells were transfected with (B) 1 μg of flag-hBST2 plasmid and an increasing amount (0.5, 1 and 2 μg) of plasmids encoding RABV G-myc, M-myc, N-myc and P-myc or (C) 1 μg of plasmids encoding individual myc-tagged RABV proteins with an increasing amount of <t>flaghBST2</t> plasmid (0.5, 1 and 2 μg). At 48 hpt, cell lysates were collected and analyzed by western blotting for RABV protein and hBST2 expression using rabbit α-myc and α-human BST2 antibodies, respectively. Arrow heads indicate glycosylated monomeric hBST2 of approximately 30 kDa. Arrows indicate a low molecular weight, glycosylated dimeric hBST2 of approximately 55 kDa. Asterisks indicate high molecular weight glycosylated dimeric hBST2 of approximately 65 kDa.
Plasmids Transfected Using Fugene 6, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+transfection+using+fugene+hd/pmc05999336-237-4-6?v=Promega
Average 90 stars, based on 1 article reviews
plasmids transfected using fugene 6 - by Bioz Stars, 2026-07
90/100 stars
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50 ng of prl-cmv control plasmid containing the luciferase gene from renilla reniformis using 3 µl fugene hd transfection reagent  (Promega)
90
Promega 50 ng of prl-cmv control plasmid containing the luciferase gene from renilla reniformis using 3 µl fugene hd transfection reagent
(A) HEK293T cells were transfected with plasmids expressing flag-hBST2. Cell lysates were prepared and incubated in the presence or absence of PNGase F and subjected to western blot analysis of hBST2. HEK293T cells were transfected with (B) 1 μg of flag-hBST2 plasmid and an increasing amount (0.5, 1 and 2 μg) of plasmids encoding RABV G-myc, M-myc, N-myc and P-myc or (C) 1 μg of plasmids encoding individual myc-tagged RABV proteins with an increasing amount of <t>flaghBST2</t> plasmid (0.5, 1 and 2 μg). At 48 hpt, cell lysates were collected and analyzed by western blotting for RABV protein and hBST2 expression using rabbit α-myc and α-human BST2 antibodies, respectively. Arrow heads indicate glycosylated monomeric hBST2 of approximately 30 kDa. Arrows indicate a low molecular weight, glycosylated dimeric hBST2 of approximately 55 kDa. Asterisks indicate high molecular weight glycosylated dimeric hBST2 of approximately 65 kDa.
50 Ng Of Prl Cmv Control Plasmid Containing The Luciferase Gene From Renilla Reniformis Using 3 µl Fugene Hd Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+transfection+using+fugene+hd/ppr0479688-186-60-69?v=Promega
Average 90 stars, based on 1 article reviews
50 ng of prl-cmv control plasmid containing the luciferase gene from renilla reniformis using 3 µl fugene hd transfection reagent - by Bioz Stars, 2026-07
90/100 stars
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plasmid transfection using fugene 6 transfection reagent kit  (Promega)
90
Promega plasmid transfection using fugene 6 transfection reagent kit
(A) HEK293T cells were transfected with plasmids expressing flag-hBST2. Cell lysates were prepared and incubated in the presence or absence of PNGase F and subjected to western blot analysis of hBST2. HEK293T cells were transfected with (B) 1 μg of flag-hBST2 plasmid and an increasing amount (0.5, 1 and 2 μg) of plasmids encoding RABV G-myc, M-myc, N-myc and P-myc or (C) 1 μg of plasmids encoding individual myc-tagged RABV proteins with an increasing amount of <t>flaghBST2</t> plasmid (0.5, 1 and 2 μg). At 48 hpt, cell lysates were collected and analyzed by western blotting for RABV protein and hBST2 expression using rabbit α-myc and α-human BST2 antibodies, respectively. Arrow heads indicate glycosylated monomeric hBST2 of approximately 30 kDa. Arrows indicate a low molecular weight, glycosylated dimeric hBST2 of approximately 55 kDa. Asterisks indicate high molecular weight glycosylated dimeric hBST2 of approximately 65 kDa.
Plasmid Transfection Using Fugene 6 Transfection Reagent Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+transfection+using+fugene+hd/pm31276789-46-5-12?v=Promega
Average 90 stars, based on 1 article reviews
plasmid transfection using fugene 6 transfection reagent kit - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


(A) HEK293T cells were transfected with plasmids expressing flag-hBST2. Cell lysates were prepared and incubated in the presence or absence of PNGase F and subjected to western blot analysis of hBST2. HEK293T cells were transfected with (B) 1 μg of flag-hBST2 plasmid and an increasing amount (0.5, 1 and 2 μg) of plasmids encoding RABV G-myc, M-myc, N-myc and P-myc or (C) 1 μg of plasmids encoding individual myc-tagged RABV proteins with an increasing amount of flaghBST2 plasmid (0.5, 1 and 2 μg). At 48 hpt, cell lysates were collected and analyzed by western blotting for RABV protein and hBST2 expression using rabbit α-myc and α-human BST2 antibodies, respectively. Arrow heads indicate glycosylated monomeric hBST2 of approximately 30 kDa. Arrows indicate a low molecular weight, glycosylated dimeric hBST2 of approximately 55 kDa. Asterisks indicate high molecular weight glycosylated dimeric hBST2 of approximately 65 kDa.

Journal: PLOS ONE

Article Title: Human BST2 inhibits rabies virus release independently of cysteine-linked dimerization and asparagine-linked glycosylation

doi: 10.1371/journal.pone.0292833

Figure Lengend Snippet: (A) HEK293T cells were transfected with plasmids expressing flag-hBST2. Cell lysates were prepared and incubated in the presence or absence of PNGase F and subjected to western blot analysis of hBST2. HEK293T cells were transfected with (B) 1 μg of flag-hBST2 plasmid and an increasing amount (0.5, 1 and 2 μg) of plasmids encoding RABV G-myc, M-myc, N-myc and P-myc or (C) 1 μg of plasmids encoding individual myc-tagged RABV proteins with an increasing amount of flaghBST2 plasmid (0.5, 1 and 2 μg). At 48 hpt, cell lysates were collected and analyzed by western blotting for RABV protein and hBST2 expression using rabbit α-myc and α-human BST2 antibodies, respectively. Arrow heads indicate glycosylated monomeric hBST2 of approximately 30 kDa. Arrows indicate a low molecular weight, glycosylated dimeric hBST2 of approximately 55 kDa. Asterisks indicate high molecular weight glycosylated dimeric hBST2 of approximately 65 kDa.

Article Snippet: HEK293T cells seeded in 10-cm 2 dishes were transfected with 6 μg of plasmid expressing RABV Gmyc and flaghBST2 complexed with Fugene ® HD transfection reagent (Promega).

Techniques: Transfection, Expressing, Incubation, Western Blot, Plasmid Preparation, Molecular Weight, High Molecular Weight

HEK293T cells were transfected with plasmids expressing RABV Gmyc and flaghBST2. At 24 hpt, cells were fixed and probed with mouse α-myc and rabbit α-human BST2 primary antibodies to detect RABV G and hBST2, respectively followed by Alexa Fluor ® 488-conjugated goat α-rabbit IgG H&L and Alexa Fluor ® 647-conjugated goat α-mouse IgG H&L secondary antibodies. Localization of the proteins were visualized by confocal microscopy.

Journal: PLOS ONE

Article Title: Human BST2 inhibits rabies virus release independently of cysteine-linked dimerization and asparagine-linked glycosylation

doi: 10.1371/journal.pone.0292833

Figure Lengend Snippet: HEK293T cells were transfected with plasmids expressing RABV Gmyc and flaghBST2. At 24 hpt, cells were fixed and probed with mouse α-myc and rabbit α-human BST2 primary antibodies to detect RABV G and hBST2, respectively followed by Alexa Fluor ® 488-conjugated goat α-rabbit IgG H&L and Alexa Fluor ® 647-conjugated goat α-mouse IgG H&L secondary antibodies. Localization of the proteins were visualized by confocal microscopy.

Article Snippet: HEK293T cells seeded in 10-cm 2 dishes were transfected with 6 μg of plasmid expressing RABV Gmyc and flaghBST2 complexed with Fugene ® HD transfection reagent (Promega).

Techniques: Transfection, Expressing, Confocal Microscopy